水分胁迫下华山松抗腐烂病的研究

A Study of the Resistance of Pinus armandii under Water Stress to Rot Disease

用不同浓度的聚乙二醇 (PEG6 0 0 0 )对华山松幼苗进行诱导水分胁迫 ,树皮的相对膨胀度 (RT值 )随PEG浓度的增大而下降 ;且树皮内的酚类物质含量、多酚氧化酶 (PPO)活性和苯丙氨酸解氨酶 (PAL)活性均有所降低 ,过氧化物酶 (POD)活性升高。说明水分状况直接影响着华山松的抗病性。4种酚类物质抑菌试验表明 ,一定浓度下酚类物质对腐烂病病原菌松黑腐皮壳 (ValsapiniFr .)菌丝体生长有抑制作用 。

The seedlings of Pinus armandii were treated with different concentration of polyethylene glycol 6000(PEG 6000).The relative turgidity(RT)and phenols content,phenylalanine ammonialyase(PAL),polyphenol oxidase(PPO)and peroxidase(POD)activities of tree barks were tested under water stress.The results showed that RT decreased with the increase of PEG concentration.Phenols content,the activities of PAL and PPO were positively correlated with RT,but negative correlation was found between POD activity and RT.The armandii pine's water condition affects directly the resistance to disease( Valsa pini Fr.) The results of phenols inhibiting Valsa pini growth were also reported.In four kinds of phenols,vanillin and pyrocatechol reduced the pathogen growth most obviously.