一氧化氮(NO)在山羊感染肝片吸虫过程中的作用

Dynamic Changes of Nitric Oxide in Serum of Goats Infected with Fasciola hepatica

8～ 1 2月龄雄性山羊 ( n=5 )人工感染肝片吸虫后 ,每周定时颈静脉采血 1次 ,用硝酸还原酶法检测血清中一氧化氮 ( NO)的动态变化 ,并观察了 NO与 Ig G抗体、嗜酸性粒细胞 ( EOS)之间的相关性。结果 ,山羊在感染后第 1周 ,NO含量明显低于对照组 ,第 2～ 6周或高或低呈波动趋势 ,自第 6周后明显高于对照组 ,其中第 7～ 8周达高峰 ,即 ( 93.73± 1 7.2 33) μmol/ L,第 7周与对照组相比 P<0 .0 1 ,第 8、1 0周 P<0 .0 5 ;Ig G抗体用间接 ELISA最早在感染后第 2周就可检出 ,并从第 4周起保持高水平 ,第 8～ 9周达到最高峰 ,血清中 NO含量与 Ig G水平的相关系数为 r=0 .5 1 1 ( P<0 .0 5 ) ,而对照组的 NO与 Ig G相关系数为 r=0 .2 5( P>0 .0 5 ) ;感染山羊在整个试验期间的 EOS明显升高 ,其中第 8周达峰值 ( P<0 .0 1 )。本试验结果表明 ,NO、Ig G和 EOS在山羊感染肝片吸虫过程中的变化趋势基本一致并呈正相关 ,且与虫体在宿主体内的移行、发育密切关联 ,显示 NO、Ig G和

Nitric oxide( NO) in sera of goats artificially infected with Fasciola hepatica was signifi- cantly changed in post-infection( PI) ,as well as Ig G in sera and eosinophil( EOS) in blood.NO level, detected by reductive enzyme assay,had a significantincrease on7th-week PI( P<0 .0 1 ) ,8,1 0 th-week PI( P<0 .0 5) ,and then kepthigherfrom 9th-week PI with a peak of ( 93 .73± 1 7.2 3 3 )μmol/ L .Ig G in the infected goats was detected by Indirect-ELISA on2 nd-week PI to be higher level than thatin the control group.NO and Ig G showed a correlationship( r=0 .51 1 ,P<0 .0 1 ) ,in the experiment group. The EOSrosehigherafterinfection of F.hepatica with a peak at6-8th postinfection.The NO,Ig G and EOSshowed a positive correlationship indicating thatthey may participate in the antiinfestation.