萤光抗体技术对鸡组织器官中赭曲霉毒素残留检测的研究

DETE RMINATION OF OCHRATOXIN A RESIDUE IN TISSUES AND ORGANS OF CHICKENS BY FLUORESCENT ANTIBODY TECHNIC

用含4.76μg/kg 赭曲霉毒素A(Ochratoxm A,简称OA)的配合饲料,饲喂30日龄的星波罗肉鸡,3日后出现明显的临床症状、10～20日内死亡,剖检后见有明显的病理变化,详见《赭曲霉毒索的毒理学试验》及《肉用鸡的赭曲霉毒素中毒》。对肝、肾、脾、心肌、肌肉、肠管等的冷冻切片及心血涂片,用自制的抗OA 抗血清作第一抗体,进行了间接免疫萤光染色,检测结果在OA 中毒鸡的各个内脏标本中均有明亮的黄绿色萤光,在试验中出现的萤光不仅说明因OA 中毒死亡的鸡体内均有毒素(被检抗原)残留,并可由此进行定性检测、而且用免疫萤光技术亦可对组织器官中的残留毒进行定位水平的检测。

The entries were 30-d-old chickens fed with mixed-feed containing 4.76μg/kgochratoxin A(OA).The chickens became diseased in 3 days,and died in 10～20 days.Theobvious pathological changes of several tissues and organs of dead chickens resulted fromOA.The residues of OA in the specimens of liver,nephridia,spleen,heart,muscle,intes-tines and blood were examined by the indirect fluorescent antibody technic and thefluorescence of several specimens was observed on a fluorescence microscope.