摘要
应用Rep -PCR分子指纹技术结合传统植物病理学分析了 5 1个采自云南各大烟区的烟草野火病菌 (Pseudomonassyringaepv.tabaci)以及其他不同致病变种、不同种、不同属的植物病原菌的群体遗传多样性。用REP ,ERIC ,BOX ,IS1112和IS 1113等 5组专化性的引物对以上菌系基因组DNA进行PCR扩增 ,并对扩增指纹图谱综合进行UPGMA聚类分析 ,在相似水平 0 .92时 ,可将云南烟草野火病菌分为 3个群 ,即YN 1,YN 2 ,YN 3。
The molecular fingerprint techniques of Rep-PCR were used to analyze the genetic diversity in a population of 51 strains of Pseudomonas syringae pv. tabaci and other genera, species and pathovars. Genomic DNA from these pathogens was amplified with 5 specific primiers, i.e. REP, ERIC, BOX, IS 1112 and IS 1113 by PCR and all the data were subjected to UPGMA cluster analysis. At a similarity level of 92%, the population of Pseudomonas syringae pv. tabaci in Yunnan was divided into 3 groups: YN 1, YN 2 and YN 3.
出处
《西南农业大学学报（自然科学版）》
CSCD
北大核心
2001年第6期514-517,共4页
Journal of Southwest Agricultural University
基金
云南省烟草公司重点项目
关键词
烟草
野火病菌
遗传多样性
云南
Pseudomonas syringae pv. tabaci
genetic diversity
