抗ω-芋螺毒素结合蛋白抗体在重症肌无力中的意义

Significance ω-Conotoxin Antibody in Myasthenia Gravis

目的:探讨ω－芋螺毒素(ω－ｃｏｎｏｔｏｘｉｎ)结合蛋白抗体在重症肌无力发病机制中的作用。方法:用ＥＬＩＳＡ方法检测３４例重症肌无力病人、２０例正常人和２１例其他神经系统疾病病人血清中抗ω－芋螺毒素结合蛋白、抗突触前膜和抗乙酰胆碱受体抗体。结果:重症肌无力病人血清中存在抗ω－芋螺毒素结合蛋白抗体,其阳性为３５．３%;突触前膜及乙酰胆碱受体抗体阳性分别为５２．９%和５０%,抗ω－芋螺毒素结合蛋白抗体与另两种抗体间有良好线性关系。结论:部分重症肌无力病人血清中存在抗ω－芋螺毒素结合蛋白抗体,提示重症肌无力是一种突触前膜,包括Ｃａ２＋通道蛋白,和突触后膜均受累及的神经肌肉接头病。

Aim:To explore the significance of ω-conotoxin binding protein antibody in the pathogenesis of myasthenia gravis(MG). Methods: ELISA was used to test the antibodies to ω-conotoxin binding protein, pre-synaptic membrane (Prsm) and acetylcholine receptor (AChR) in sera from 34 MG patients, 20 healthy controls and 21 other neurological disorders (OND). Results: Antibodies to ω-conotoxin binding protein were found in 35.3% of MG patients and the antibodies to Prsm and AChR were found in 52.9% and 50% of patients with myasthenia gravis respectively, ω-conotoxin binding protein antibody was well related to other two antibodies. Conclusion :ω-conotoxin binding protein antibody do exist in some MG patients. This result demonstrates that MG is a disease caused by damage of neuro-muscluar conjunction. Both pre-synaptic and post-synaptic membrances including Ca2+ channel protein are involved in the pathogenesis.