毛冠鹿p16^(INK4)基因第二外显子序列分析

Analysis of p16^(INK4) Gene Exon 2 in Elaphodus Cephalophus

采用PCR扩增技术首次克隆毛冠鹿p16 INK4 基因第二外显子 ,DNA杂交和序列分析发现 ,在 30 7个碱基的第二外显子中仅有 5个碱基的差异 ,同源性达 98.4% .p16 INK4 基因的第二外显子是高度保守的区域 。

The DNA fragment containing exon 2 of p16 INK4 gene was cloned by PCR method from Elaphodus cephalophus genomic DNA.Southern blotting and sequencing analysis showed there was very high homology (98.4%) of p16 INK4 gene exon 2 between them.The exon 2 region is very conservative ,it plays an important role in the function of p16 INK4 gene.