摘要
采用PCR扩增技术首次克隆毛冠鹿p16 INK4 基因第二外显子 ,DNA杂交和序列分析发现 ,在 30 7个碱基的第二外显子中仅有 5个碱基的差异 ,同源性达 98.4% .p16 INK4 基因的第二外显子是高度保守的区域 。
The DNA fragment containing exon 2 of p16 INK4 gene was cloned by PCR method from Elaphodus cephalophus genomic DNA.Southern blotting and sequencing analysis showed there was very high homology (98.4%) of p16 INK4 gene exon 2 between them.The exon 2 region is very conservative ,it plays an important role in the function of p16 INK4 gene.
出处
《南京师大学报(自然科学版)》
CAS
CSCD
2001年第3期80-82,87,共4页
Journal of Nanjing Normal University(Natural Science Edition)
基金
江苏省教育厅高校科研基金 (99KJB180 0 0 4)