白血病细胞对三氧化二砷的不同敏感性

Different sensibility of leukemic cells to arsenic trioxide

目的 :探索白血病细胞对三氧化二砷 (As2 O3 )敏感性的差异。方法 :用台盼蓝染色区分死活细胞 ,检测细胞活性 ;利用碘化丙啶 (PI)染色和流式细胞技术测定凋亡细胞和细胞DNA的含量。结果 :0 5 0 μmol/L以上浓度的As2 O3 与细胞共同孵育 4 8h以后对NB4细胞有明显的杀伤作用 ;而对K5 62细胞来说 ,As2 O3 浓度要达到 2 .0 μmol/L以上 ,72h以后才有明显的杀伤作用。 结论 :白血病细胞对As2 O3 有不同的敏感性。

Aim: To investigate and study the sensibility of leukemic cells to arsenic trioxide. Methods: Histochemistry, propidium iodile and flow cytometry were used in the experiments. Results: More than 0. 5μmol/L of arsenic trioxide for 48 h could produce the apoptosis to NB4 leukemic cells; however, 2μmol/L of arsenic trioxide for 72 h could produce the apoptosis to K562 leukemic cells. WTHZConclusion: WTBZIt was discovered that there exists the different sensibility of leukemic cells to arsenic trioxide.