基于BioMEMS技术的DNA芯片研究

DNA CHIPS RESEARCH BASED ON BioMEMS TECHNOLOGIES

近年来基于微电子机械系统 (MEMS)技术研究和开发的一个新领域就是BioMEMS ,特别是对生物芯片和生物化学微分析系统的研究。文中叙述了基于BioMEMS技术的DNA芯片研究 ,介绍了几种不同结构的DNA芯片 ,以及DNA PCR扩增器芯片、DNA CE毛细管电泳芯片、微流动控制器件和实验室芯片 (LabonaChip)等。还报告了作者们成功采用MEMS技术 ,研制成集成化微结构型PCR DNA扩增芯片 ,芯片上包括有加热器、温度传感器、2 μL容积的反应池以及输入输出通道 ;能实现快速扩增 ,最快加热速度为 15℃ s ,降温速度为 10℃ s。研制成Si—玻璃 ,玻璃—玻璃和玻璃—PDMS三种结构的毛细管电泳芯片 ,沟道宽 2 0 0 μm ,深 2 0 0 μm。研制成一种表面等离子体谐振 (SPR)生化分析系统 ,其入射光扫描角范围从 40°到 70° ,分辨率 0 .1%度 ,可测折射率范围 1.0 4～ 1.47。

In recent years a new research and development field based on micro-electro-mechanical system (MEMS) technology is BioMEMS, especially regarding DNA chip and micro biochemical analysis system. This paper describes several structures DNA chips, such as several different functions micro chips: micro polymerase chain reaction, micro capillary electrophoresis chip, and microfluidic control devices and lab on a Chip. In this brief paper we have reported successfully made an integrated microstructure PCR-DNA amplification chip based on MEMS technology, which includes a heater, a temperature sensor, a 2 μL reaction chamber, an inlet channel and an outlet channel. The PCR chip can rapidly fulfill DNA-amplification. The maximal heating speed is over 15°C/s and cooling speed is 10°C/s. The Si-glass, glass-glass and glass-PDMS capillary electrophoresis chips with 200 μm wide and 200 μm deep channels are fabricated. A novel surface plasma resonance (SPR) biochemical analysis system is presented, which has scanning angle from 40 degree to 70 degree, angle scanning resolution 0.001 degree and measurement refractive index range from 1.04 to 1.47.