油菜扩繁中茎段长度对腋芽生长发育的影响

Effect of Lengths of Stem Segments on Growth and Development of Axillary Buds in Asexual Reproduction in B.napus

以甘蓝型油菜 97TZ1 92的单株无性系试管苗为材料 ,探讨了油菜茎段培养中腋芽生理学下端茎切段长度对腋芽生长发育的影响。结果表明 ,培养 2 4h后 ,有 80 .7%的茎段从叶腋露出幼小生长点 ;5 d后已有93 .2 %茎段出芽。但培养过程中 ,随着茎切段长度的减小 ,幼芽生长发育明显减慢 ,正常率和成活率降低。培养 3 0 d后统计 ,幼苗平均苗高和生根率降低 ,平均每株幼苗的根数、根长以及叶片数也出现减少的趋势 ,其中茎段长度以 1 mm≤ x<4mm的变化较明显。因此认为 ,茎切段培养中叶腋生理学下端茎切段长度至少应大于 4～ 6mm。

Studied the effect of stem segment lengths of physiological low parts of axils on growth and development of axillary buds in the stem culture with somaclonal tube shoots of plant 97TZ192 in Brassica napus .The result showed that 80.7% of stem segments sprouted at axils in 24 h,93.2% emerged in 5 d.But growth and development of the sprouts were obviously slow,and the rate of living buds and the rate of normal buds were also low while length of stem segments were short in culture.And statistics in 30 d culture,the average shoot height and the rate of rooting turned low. Number of roots,length of roots and number of leaves of each shoot turned to reduce,too.The change was more obvious in stem length of 1 mm ≤ x < 4 mm than in 4 mm ≤ x < 6 mm .So stem segment lengths of physiological low parts of axils shoud be more than 4~6 mm in culture of stem segments.