高温高湿环境犬肢体火器伤后肿瘤坏死因子-α含量变化的实验研究

Dynamic changes of TNF-αcontent in dogs after gunshot wound in the limbs in hot and humid environment

目的探讨高温高湿环境下肢体火器伤后肿瘤坏死因子-α(TNF-α)含量变化的规律。方法将犬随机分为常温常湿组、热习服组、高温高湿组,分别在致伤前及火器伤后1、3、4、6、8、10、14、18、24h检测血浆和伤道骨骼肌中TNF-α含量。结果(1)伤道骨骼肌中TNF-α含量较血浆中明显升高(P<0.05);(2)常温常湿创伤组TNF-α4h开始升高,14h达高峰;(3)高温高湿创伤组1h升高,10h达到高峰,其峰值显著高于常温常湿组(P<0.05);(4)热习服组TNF-α变化基本和高温高湿组一致,只是升高幅度较低(P<0.05)。结论(1)TNF-α参与了高温高湿环境下肢体火器伤后机体的病理生理过程;(2)预示着TNF-α是高温高湿环境下火器伤病程变化的指标。

Objective To investigatethedynamicchangesof TNF-αcontentin theplasmaandbodytissuesof dogs after gunshotwoundinthelimbsinhotandhumidenvironment.Methods Eighteendogswithgunshotwoundweredividedinto3groups(6in each),one observedin normalenvironment(NE group)andtheothertwo in hot andhumidenvironment includinga heat-acclimatizedgroup(HAgroup)anda non-acclimatizedgroup(NHAgroup).Thecontentsof tumornecrosis factor-α(TNF-α)inthe plasmaand muscletissuesfromthegunshotwoundtractweremeasuredat0,1,3,4,6,8,10,14,18,24h respectivelyaftertheinjury.Results TNF-αcontentinthe musculartissuefromgunshotwoundtractwas significantly higherthanthatintheplasma(P<0.05).At4h afterinjury,TNF-αcontentstartedto riseinNEgroup,reachingthepeaklevel at14h,theriseandpeakingof whichoccurredat3and10h respectivelyin NHE groupwithhigherpeaklevelthanthatin NEgroup(P<0.05).Thechangesof TNF-αcontentinHAgroupwas consistentwithNHE groupexceptfora significantly lower peaklevel(P<0.05).Conclusion TNF-αparticipatesin thepathophysiologicalprocessafter gunshotwoundin hotand humidenvironmentandmayplaytheroleof an indicatorforthepathologicalchangesthattakeplaceaftertheinjury.