摘要
目的观察重组人内皮抑素(rhES)对小鼠肺腺癌LA795生长和转移的抑制作用。方法对ThES高效表达克隆pCX的表达产物进行纯化,得到ThES。将LA795肺腺癌细胞接种于T739小鼠背部皮下,随机分成2组,接种后第10天起分别给予rhES20mg/kg·b.w.或等体积PBS缓冲液皮下注射,1次/d,共14d。观察2组肿瘤生长情况、肺湿重、肺表面转移结节数、动物生存时间,分别进行t检验。结果治疗前后rhES组肿瘤体积分别为(650±201)mm3、(1642±211)mm3;而PBS组肿瘤体积由治疗前的(623±248)mm3增至(9194±952)mm3。rhES组肺湿重、肺表面转移结节数明显少于PBS组,动物生存时间明显延长(P<0.01)。结论rhES可明显抑制LA795所致的小鼠实验性肿瘤的生长与转移,延长动物的生存时间。
Objective To evaluatetheinhibitoryeffectof recombinanthumanendostatinon tumorgrowthandmetastasisof ade-nocarcinomaLA795inmice.Methods Recombinanthumanendostatinwas purifiedfromendostadin-expressingpCXclones.LA795cellswereinoculatedsubcutaneouslyon thebackof T 739 mice,whichwererandomizedinto2groups.Fromthetenth dayon,treatmentgroupwas given20mg/kgrecombinanthumanendostatinsubcutaneouslydailyfor14consecutivedays,and thecontrolgroupreceivedPBSinthesamemanner.Thesizesof thesubcutaneoustumors,lungweights,thenumberof metas-tasesoverthelungsurfaceandthesurvivaltimeof themicewereobserved.Results Thetumorsizesof thetreatmentgroupin-creasedslowlyfrom(650±201)mm 3 to(1642±21)mm 3 whencomparedwiththoseof thecontrolgroupwhichshowedan in-creasefrom(623±248)mm 3 to(9194±952)mm 3 .Thelungweightof the2groupswas(190±25)mg and(324±43)mg respec-tively,andthenumberof lungsurfacemetastaseswas8±2and22±8foreach.Theaveragesurvivaltimeof theratsinthe2groupswas48d and27d,respectively.Allparametersmeasuredbetweenthe2groupsshowedsignificantdifferences(P<0.01).Con clusion Recombinenthumanendostatinhasstronginhibitoryeffecton boththegrowthof primarytumorandmetastasisof lungadenocarcinomaLA795cells,andprolongsthesurvivaltimeof thetumor-bearingmice.
出处
《第一军医大学学报》
CSCD
北大核心
2001年第12期917-919,F003,共4页
Journal of First Military Medical University
基金
南方医院院长科研基金
