善宁对大鼠肝部分切除术后种植性肝癌作用的实验研究

Effects of octreotide on implanted liver tumor after partial hepatectomy in rats

目的 探讨生长抑素类似物善宁对肝部分切除术后种植性肝癌的作用及其相关机制。 方法 以Wistar大鼠种植性肝癌模型为研究对象 ,将 6 4只雄性Wistar大鼠随机分为 4组 ,每组 16只。A组行单纯右肝叶肿瘤种植 ,B、C、D组均行左肝叶切除加右肝叶肿瘤种植。C、D组分别为术后12h、72h开始首次善宁治疗组 (5 0ug/kg,腹腔内注射 ,每日 2次 )。A、B组为对照组 (生理盐水 ,方法与剂量同上 )。 结果 术后 1周 ,A组肿瘤体积明显小于B、C、D组 (P <0 0 1)。C、D组的肿瘤体积明显小于B组 (P <0 0 1) ,而肿瘤细胞的凋亡率则明显高于B组 (P <0 0 1)。C组与D组的肿瘤体积和细胞凋亡率之间亦存在显著差异 (P <0 0 1)。 结论 善宁可以抑制术后肝肿瘤的生长。

Objectives To investigate the effects of octreotide(OCT) on experimental live tumor after partial hepatectomy (PH) in rats and explore its intrinsic mechanism. Methods Sixty-four male rats were randomly divided into four groups and operated on with laparotomy. Walker-256 tumor was implanted in all the four groups. Group A served as the control, and groups B, C and D received left lobe PH. Octreotide at a dose of 50 μg/kg was intraperitoneally(IP)administered twice a day in groups C and D (OCT). The time of the first administration was at the 12th hour after PH in group C and the 72nd hour after PH in group D. The rats in groups A and B received a similar volume of normal saline (NS). Results Group A showed a significant reduction of implanted liver tumor volume at the 7th day versus group B (P<0.01). Groups C and D showed a significant reduction of tumor volume at the 7th and 15th day versus group B(P<0.01). The tumor cell apoptotic rate was significantly higher in groups C and D than in groups A and B at the 7th and 15th day(P<0.01). Conclusion The growth of experimental liver tumor is enhanced after PH bat can be inhibited by OCT.