微孔过滤实验中红细胞变形性的表达

The Expression of Red Blood Cell Deformability in Micropore Filtration Tests

在用微孔过滤法测定红细胞变形性的实验中 ,为了便于定量地评定和比较红细胞变形性 ,我们对目前常用的表达变形性的各种物理参数和人为定义的指标的确切含义和适用性进行了较为全面的分析和讨论。认为红细胞平均过孔时间 (或相对过孔阻力 )和堵孔红细胞比例分别用于表达红细胞平均变形性和堵孔特性比较合适。早期研究力求避免过滤过程中红细胞堵塞膜孔现象对实验的干扰 ,但通过对近年来不同实验方法下实验结果的分析 ,发现红细胞堵孔特性蕴涵着重要信息 ,反映了红细胞变形性的不同方面。表达这一特性的参数对一些物理、化学 ,尤其是病理因素引起的变形性改变 。

For the purpose of evaluating and comparing RBG deformability quantitatively with micropore filtration method, the precise meanings and applicability of physical parameters and defined indices expressing RBC deformability are discussed and analyzed in detail. The mean transit time (or the relative transit resistance) and the proportion of pore-clogging cells are found to be appropriate for representing the average deformability and the clogging characteristic of RBCs respectively. To eliminate the effects of clogging on filtration, previous studies were conducted filtration with as less dogging as possible. Investigations on experimental results from various filtration methods, however, reveal that the clogging property of RBCs contains important information on the RBC deformability. It reflects a different aspect of the RBC deformability besides the average deformability and is more sensitive to the abnormal deformability induced by physical, chemical, especially pathological factors.