9-(4-乙氧羰基苯氧基)-6,7-二甲氧基-1,2,3,4-四氢吖啶盐酸盐对PC12细胞缺氧缺血损伤的保护作用

Protective effects of 9-(4-ethoxycarboxylyphenoxy)-6,7-dimethoxy-1,2,3,4- tetrahydro acridine on anoxia and ischemic injury in cultured PC12 cells

目的 研究 9 (4 乙氧羰基苯氧基 ) 6 ,7 二甲氧基 1,2 ,3,4 四氢吖啶盐酸盐 (EDT)对神经细胞缺血缺氧损伤的影响。方法 离体培养的PC12细胞 ,用连二亚硫酸钠造成缺氧 /复氧损伤模型 ,用NaCN加缺糖造成拟缺血损伤模型 ,通过MTT微量比色、培养介质LDH活力测定研究EDT对两模型的保护作用。结果 在 10 - 8～ 10 - 6mol·L- 1范围内 ,EDT浓度依赖地降低两种损伤所致培养介质内LDH的释放 ,增加MTT比色值 ,同时 10 - 6mol·L- 1EDT对两模型的保护作用具有时间依赖性 ,4 8h达效应平台。结论 EDT对PC12细胞缺血缺氧损伤具有保护作用。

Aim To investigate the effects of EDT on anoxia and ischemic injury in cultured PC12 cells. Methods Cultured PC12 cells were treated with 1 mmol·L -1 Na 2S 2O 4 and 20 mmol·L -1 NaCN in combination with glucose deprivation. The protective effects of EDT on these two models were evaluated by lactate dehydrogenate (LDH) efflux assay and colormetric MTT assay.ResultsEDT, within the range of 10 -8～ 10 -6 mol·L -1, significantly antagonized LDH efflux induced by two models and increased the optical density at 570 nm tested by colorimetric MTT assay in concentration-dependent manner. 10 -6 mol·L -1 EDT might time-dependently inhibit two injuries and reach maximal level at 48 h. Conclusion EDT can protect PC12 cells from anoxia and ischemic injury.