Co-H_2O_2-3,5-二溴水杨基荧光酮催化分光光度法测定痕量钴

DETERMINATION OF TRACE COBALT(Ⅱ) BY CATALYTIC SPECTROPHOTOMETRY

目的 :研究测定痕量钴的新体系。方法 :催化分光光度法。结果 :在NaOH介质中 ,Co(Ⅱ )对H2 O2 氧化 3,5 -二溴水杨基荧光酮 (DBSF)的退色反应有强烈催化作用。据此建立了测定痕量钴的动力学新方法 ,该法检出限为 8.7× 10 -13 g/ml,线性关系 0～ 4ng/ 10ml。 结论 :本方法灵敏度高 ,选择性好 ,可直接测定VB12

Objective:To study a new system of determining trace cobalt(Ⅱ).Methods:Catalytic spectrophotometry.Results:Cobalt(Ⅱ) in NaOH can catalyze the discolouring oxidation of 3,5-dibromosalicylfluorone(DBSF) by H 2O 2,a new method for determining trace cobalt(Ⅱ) was established.The detection limit of the method is 8.7×10 -13 ?g/ml and the range of determination is 0～4?ng/10?ml.Conclusion:The method has high sensitivity and selectivity.Satisfactory results were obtained for the determination of trace cobalt(Ⅱ) in VB 12 .