维生素K_2抑制MGC-3细胞生长的实验研究

Inhibition of gastric cancer cells MGC80-3 by vitamin K_2

目的 探讨维生素K2 (VK2 )抑制肿瘤细胞生长的机制。方法 以人低分化胃腺癌细胞系MGC 80 - 3为靶细胞 ,应用MTT法检测VK2 的生长抑制作用 ,并以流式细胞仪、荧光显微镜、电镜和DNA凝胶电泳技术等研究细胞调亡形态学改变和凋亡率。结果 10 μmol/L及以上浓度的VK2 处理 48h可显著抑制MGC 80 - 3细胞生长 (P <0 .0 1) ,细胞呈明显的形态学改变 :细胞皱缩 ,染色质凝集 ,分布于核膜内缘 ,凋亡小体形成 ,基因组DNA片段化 ,胞内RNA含量下降 ,凋亡细胞百分率呈药物浓度依赖性。结论 VK2 能有效地抑制MGC80 - 3细胞生长 。

Objective To study the mechanism of vitamin K 2 inhibition of gastric cancer cells.Methods Human gastric mucoid adenocarcinoma cell line (MGC80-3) was used as target cells for the effect of Vitamin K 2.Growth inhibition was analyzed by MTT assay.Cell apoptosis was evaluated by fluorescein microscopy,transmission eletron microscopy and agarose gel electrophoresis.Results Treatnent with vitamin K 2 at concentration of 10 μmol/L and higher remarkably inhibited growth of MGC80-3 cells.Further study showed that vitamin K 2 induced MGC80-3 cell death characterized by apoptotic phenotypes,chromatin DNA fragmentation and DNA content changes.Vitamin K 2 induced apoptosis of MGC80-3 cells in a dose- dependent manner.Conclusions Vitamin K 2 efficiently induces apoptosis in one of human gastric cancer cell lines,suggesting that it is a promising potential therapeutic agent in the treatment of human gastric cancer.