ELISA检测颅脑损伤患者血清S100B蛋白变化及其意义

The changes and significance of serum S100B protein in patients with brain injury

目的 :探讨颅脑损伤患者血清S10 0B蛋白动态变化及其临床意义。 方法 :用双抗体夹心ELISA法检测10 0名健康人和 12例颅脑损伤患者血清S10 0B蛋白水平 ,同时观察血清S10 0B蛋白动态变化。 结果 :12例颅脑损伤并发脑挫裂伤患者在伤后 12h血清S10 0B水平开始升高 (P <0 .0 0 1) ,2 4h即达高峰。轻、中型患者治疗后S10 0B蛋白可降至正常水平 ,而重型患者仍维持高水平 ,而且病程迁延。 结论 :血清S10

Objectives:To investigate the time changes and the clinical significance of S100B protein in serum from patients with brain injury. Methods:A double antibody sandwich ELISA was used to measure the S100B protein in serum from 100 controls and 12 patients with brain injury.Meanwhile,the time difference were observed in 12 cases. Results:The concentrations of S100B in serum increased during the first 12 hours( P <0.001) and reach a maximum at 24 hours.The level of serum S100B protein in patients with slightly or moderately brain injury fell to normal level after treatment while a higher level of serum S100B protein were found in the whole course in the patients with severely brain injury. Conclusions:The serums S100B protein can be a new index for the early diagnosis and be used to estimate the grades and prognosis of brain injury.