RP-HPLC法测定酸枣仁中黄酮碳苷的含量

Quantitative determination of spinosin in seed of Ziziphus jujuba var. spinosa by RP-HPLC

目的 建立测定酸枣仁中黄酮碳苷含量的新方法。方法 RP- HPL C法 ,采用 Hypersil- C1 8柱为分析柱 ,乙腈 -水 (17∶ 83)为流动相 ,检测波长为 334 nm,,流速为 0 .8m L/ min。结果 黄酮碳苷在 5 .2 80～ 14.0 8μg/ m L范围内与峰面积呈良好的线性关系 (r=0 .9997,n=6 ) ,平均回收率为 95 .8%。结论 该法简便、快速、重现性好 ,适用于酸枣仁中黄酮碳苷的定量分析。

Object To develop a new method for the quantitative determination of spinosin in the seed of Ziziphus jujuba Mill. var. spinosa (Bunge) Hu ex H. F. Chou Methods The determination was carried on RP-HPLC, using Hypersil-C 18 column, acetonitrile∶water (17∶83, v/v) as mobile phase with a flow rate of 0 8 mL/min and detected at the wave length of 334 nm Results The calibration curve was linear in the range of 5 280～ 14 08 μg/mL The average recovery rate was 95 8% Conclusion The method is appropriate for the quantitative determination of spinosin