L-精氨酸对阻塞性黄疸大鼠单核吞噬细胞系统功能的影响

Effect of L-arginine on phagocytic function of mononuclear phago cyte system in rat with obstructive jaundice

目的 探讨L 精氨酸 (L Arg)对阻塞性黄疸大鼠单核吞噬细胞系统 (MPS)吞噬功能的影响。方法 将SD雄性大鼠 5 4只随机分为 3组 :假手术对照组 (SO) ;胆总管结扎组 (BDL) ;胆总管结扎 +L 精氨酸组 (BDL +L Arg) ,每组术后又分设 7、14、2 1d 3个时间点检测MPS和枯否细胞 (KC)的吞噬功能 ,并测定肝组织丙二醛 (MDA)和NO2 -/NO3-含量。结果 与SO组比较 ,BDL组各时间点MPS吞噬功能 (P <0 .0 1)和KC吞噬功能 (P <0 .0 5 )明显减弱。BDL +L Arg组在胆管结扎 7、14d时MPS和KC吞噬功能明显强于BDL组 (P <0 .0 5 ) ,2 1d时两组MPS和KC吞噬功能差异无显著性 (P >0 .0 5 ) ,但与SO组相比 ,BDL +L Arg组各时间点MPS吞噬功能仍明显降低 (P <0 .0 5 )。BDL +L Arg组在胆管结扎 7、14d时血清转氨酶和肝组织MDA含量显著低于BDL组 (P <0 .0 5 ) ,而NO2 -/NO3-含量显著高于BDL组 (P <0 .0 1)。结论 L 精氨酸有减轻阻塞性黄疸时肝脏损伤和增强MPS、KC吞噬功能的作用 ,但存在一定时效关系。

Objective To investigate the effect of L -arginine on phagocytic function of mononuclear phagocyte system in rat with ob structive jaundice.Methods Fifty-four male SD rats were randomly divided in to three groups: sham-operation (SO) group,bile duct-ligation (BDL) group a nd bile duct-ligation +L-arginine group (BDL+L-Arg),and each group were rand omly divided to subgroups of day 7,14,21 again.The phagocytic functions of MPS and KC were evaluated on the day 7,14 and 21,and hepatic malondialdehyde (MDA ) and NO_2^-/NO_3^- contents were examined.Results The phagocytic functions of MPS and KC were remar kably decreased at each time point in BDL group,but were significantly enchance d by injecting L-arginine on the day 7,14(P<0.05).However,the enhanceme nt disappeared on the day 21(P>0.05).Moreover,the phagocytic function of MPS in BDL+L-Arg group was significantly lower at each time point than in the SO group (P<0.05).Serum transaminase and hepatic MDA contents were lower i n th e BDL+L-Arg group than in the BDL group on the day 7,14(P<0.05).In contr ast,hepatic NO_2^-/NO_3^-content was higher in the BDL+L-Arg group (P <0.01).Conclusion L-arginine can ameliorate the liver injury an d enhance the phagocytic functions of MPS and KC,which are related to the time that biliary obstruction lasts.