动力学涂层毛细管电泳分离双链脱氧核糖核酸片段

Separation of Double Strand Deoxyribonucleic Acid Fragments Using Dynamic Coating Capillaries

以异丙醇为聚合反应链转移试剂，水相法合成了短链聚Ｎ，Ｎ－二甲基丙烯酰胺（ＰＤＭＡ）。研究表明该聚合物能在毛细管内壁形成稳定的动力学涂层，从而有效地抑制电渗流和毛细管内壁与ＤＮＡ的作用。这种介质被成功地应用于ＤＮＡ片段的高效分离。

A simple method was developed to synthesize the short-chain poly (methylacrylamide) (PDMA) using isopropanol as a chain transfer agent. This novel polymer can form a dynamic coating on the inner surface of the capillary, thereby suppressing the electroosmotic flow and deoxyribonucleic acid (DNA )-capillary wall interaction. Highly-efficient and reproducible separation of double strand DNA fragments were obtained in a bare fused silica capillary using PDMA as a separation medium.