膀胱癌血管形成与vEGF表达的研究

Study on angiogenesis and expression of vascular edothelial growth factor in bladder carcioma

目的 :探讨膀胱癌血管内皮生长因子 (vEGF)表达和肿瘤血管形成之间的关系及临床意义。方法 :采用免疫组织化学、原位杂交方法检测 4 2例膀胱癌组织的vEGF的表达 ,Ⅷ因子相关抗原染色定量微血管密度 (MVD)。结果 :①vEGFmRNA原位杂交显示膀胱癌肿瘤表达mRNA而内皮细胞不表达。且在不同组织学类型之间有差异性 (P <0 0 5 )。②膀胱癌组织中MVD在不同组织类型之间有显著差异性 (P <0 0 5 )。③vEGFmRNA原位杂交不同结果之间MVD有显著差异性 ( P <0 0 5 )。结论 :vEGF是肿瘤血管形成的重要因素 ,检测其表达以及计数MVD可作为预测肿瘤的生长、发展、浸润、转移、预后的指标 。

Objective:To study the relationship between MVD and the expression of vEGF and to evalute their clinical significance Methods:Immunohistochemistry and hybridization in situ were used to study the expression of vEGF and Ⅷ factor related antigen in bladder carcinoma Results:There was significant difference of expression of vEGFmRNA according to different tissue types Significant difference of MVD were shown in different results of hybridization Conclusions:vEGF is important factors on tumor angiogenesis Expression of MVD and vEGF mRNA were related to biological action of bladder carcinoma and has important clinical significance