原发性膀胱移行细胞癌P16基因缺失研究

Significance of Study on P16 Gene Loss in Primary Transitional Cell Carcinomas of Urinary Bladder

目的 :探讨原发性膀胱移行细胞癌中P16基因缺失及P16蛋白的表达。方法 :应用PCR和免疫组化方法对 38例膀胱移行细胞癌标本P16基因缺失和P16蛋白的表达进行分析。结果 :原发性膀胱移行细胞癌P16基因缺失率为 18.42 % ,缺失率明显低于培养的膀胱癌细胞系 ;P16蛋白的阳性表达率为 5 2 .6 3%。结论 :肿瘤细胞自原体转入培养环境的适应阶段P16基因缺失可能有助于肿瘤细胞的增殖 ,在膀胱肿瘤的发生中起重要作用。

Objective:To explore the loss of P16 gene and expression of P16 protein in primary transitional cell carcinomas(TCCs) of bladder.Methods:PCR and immunohistochemical methods were used to detect the loss of P16 gene and expression of P16 protein in 38 specimens of primary TCCs of urinary bladder.Results:The loss rate of P16 gene was 18.42% in 38 TCCs specimens and the positive rates of P16 expression were 52.63%. Conclusion:There are apparently an lower frequency of P16 loss and mutation in primary bladder cancer compared with the culture tumor counterparts.The loss of P16 gene confers a selective growth advantage during adaptation of tumor cells to culture and hence is probably important in the generation of bladder cancer cell lines.The loss of P16 gene makes a significant contribution to bladder cancer oncogeness.