胃肠癌患者外周血T细胞及NK细胞免疫功能状况的实验研究

The Study of T Lymphocyte and NK Cell Immune Function in the Patients with Gastrointestinal Cancer

目的 :研究胃肠癌患者外周血T细胞及NK细胞免疫功能状况。方法 :免疫细胞化学方法检测 49例胃肠癌患者外周血T细胞及NK细胞。结果 :与正常人相比 ,胃肠癌患者外周血中CD3+ 、CD4+ 细胞数明显减少 (P <0 .0 1) ,CD8+ 细胞数明显增多 (P <0 .0 5 ) ,CD4+ CD8+ 比值明显降低 (P <0 .0 1) ;NK细胞数量明显减少 (P <0 .0 5 )。胃肠癌患者的细胞免疫状态与肿瘤的TNM分期呈负相关 (P <0 .0 5 ) ,病期越晚 ,患者的免疫损害越严重。结论 :胃肠癌患者细胞免疫功能低下 ,对肿瘤患者T细胞及NK细胞进行监测 ,有利于了解病情 ,为肿瘤的免疫增强治疗提供参考。

Objective:To study T lymphocyte and NK cell immune function in the patients with pastrointestinal cancer.Methods:Using immunocytochemical stain method.Results:The percentages of CD3 +,CD4 +,CD57 + cells and CD4 +/CD8 + ratio in patients with gastrointestinal cancer were significantly lower than those in the control group (P<0.05).The percentage of CD8 + cells was higher than that in the control group(P<0.05).The cellular immune status of the patients with gastrointestinal cancer was negatively related to the TNM stages of the disease(P<0.05).The higher the stage of the disease was,the more severe the patient's immunity damage was.Conclusions:The cellular immune function of patients with gastrointestinal cancer patients was low.T cell subgroup and NK cell measurements may help determine the disease stages and offer reference to tumor immunotherapy.