PCNA、P53、CerbB-2及bcl-2在胆囊癌中的表达

PCNA、P53、CerbB－2 and bcl－2 Expression in Gallbladder Carcinoma

目的 观察胆囊高分化癌与低分化癌的生物学特性，了解胆囊癌的肿瘤细胞分化与预后的关系。 方法 用Envision免疫组化二步染色方法，检测40例胆囊癌（高分化癌24例，低分化癌16例）PCNA、P53、CerbB－2、bcl－2的表达情况。 结果 PCNA、P53、CerbB－2在胆囊癌中均有过度表达，其中P53在胆囊高分化癌与低分化癌之间有显著差异，P＜0．05；半定量积分分级的P53和CerbB－2在胆囊高分化癌与低分化癌间有显著差异，P＜0．05。 结论 胆囊癌细胞增殖都非常活跃胆囊高分化癌的CerbB－2过度表达明显，而胆囊低分化癌的P53过度表达显著。在本组试验中，胆囊癌的预后与肿瘤分化程度无明显相关。

 Objective to investigate the relationship between differentiated and prognosis of gallbladder carcinoma from observing the biological character of well－differentiated and poorly－differentiated gallbladder carcinoma． Methods PCNA P53 CerbB－2 and bcl－2 gene expressions were detected with The Envision immunohistochemical method in 40 cases of gallbladder carcinoma (24 cases of well differentiation and 16 cases of poor differentiation)． Results The overexpression positive rates of P53 gene were related to well－differentiated and poorly－differentiated gallbladder carcinoma P＜0．05． Score grade of P53 and CerbB－2 gene expressions were related to well－differentiated and poor differentiated gallbladder carcinoma． Conclusion The gallbladder carcinoma cell proliferate very actively． Over expression of the CerbB－2 were obvious in well－differentiated gallbladder carcinoma and over expression of the P53 were distinct in poorly differentiated gallbladder carcinoma． It was relation between CerbB－2 and P53． The prognosis and differentiation of gallbladder carcinoma were unrelative． 