HLA-C与湖北地区汉族寻常型银屑病的相关性

A Study of the Correlation between HLA-C and Psoriasis Vulgaris of Han Nationality in Hubei Province

目的 :探讨寻常型银屑病 (psoriasisvulgaris,PV)与人类白细胞抗原 (humanleukocyteantigens,HLA)复合体C基因座位第 73位氨基酸密码子的相关性。方法 :采用聚合酶链反应 (PolymeraseChainReaction ,PCR)———变性聚丙烯酰胺凝胶电泳—DNA银染、测序 ,检测了 10 4例PV患者和 110例健康对照者的HLA C基因第 73位氨基酸密码子。结果 :PV患者编码HLA C抗原第 73位氨基酸的核苷酸 ,为丙氨酸序列 (73alaninesequence,73 Ala)的频率比健康对照组显著性增高 (81.7%比 5 3.6 % ,RR =3.78,χ2 =19.2 1,P <0 .0 0 5 ) ;为苏氨酸序列 (73threoninesequence,73Thr)的频率比健康对照组显著性降低 (77.9%比 89.1% ,RR =0 .43,χ2 =4.92 ,P <0 .0 5 )。 4个PCR产物测序结果证实扩增片段确实为所需目的DNA。结论 :HLA C第 73位密码子与PV发病率存在相关性 ;HLA C的73Ala可能是PV的易感因素 ,而其73Thr似乎是PV的保护性因素 ,且为银屑病病因及发病机制的探讨提供了一些信息。

Objective:To explore the correlation between codons at position 73 of HLA C and psoriasis vulgaris for people of Han nationality in Hubei Province.Methods:Polymerase Chain Reaction (PCR)—denature polyacrylamide gel electrophoresis—DNA silver stain technique and DNA sequencing for PCR products were employed to detect the codons at position 73 of HLA C in 104 cases with psoriasis vulgaris and 110 healthy controls.Results:The frequency of nucleotide sequence coding alanine at position 73 of HLA C antigens( 73 Ala) in the cases was 81.7%,while that in healthy controls was 53.6%.The former was significantly higher than the latter (RR=3.87,χ 2=19.12,P<0.005).But the frequency of nucleotide sequence coding threonine at position 73 of HLA C antigens ( 73 Thr) in PV patients was significantly lower than that in healthy controls (77.9% versus 89.1%,RR=0.43,χ 2=4.92,P<0.05).DNA sequencing for four PCR products ascertained that the PCR products were the DNA fragments which were needed.Conclusions:In PV patients of Han nationality in Hubei Province, 73 Ala of HLA C gene may play a role in susceptibility to PV,but 73 Thr of HLA C gene seems to be a resistant factor to PV.This study provides some message for exploring etiology and pathogenesis of PV.