机械通气对新生儿血浆中抗氧化酶活性的影响

The Effect of Mechanical Ventilation on Antioxidant Enzyme Activities in Neonatal Blood Plasma

目的 探讨机械通气对新生儿血浆中抗氧化酶 (antioxidant enzyme,AOE)活性的影响。 方法 用酶活性测定法检测 72例新生儿机械通气前后血浆中过氧化氢酶 (catalase,CAT)、谷胱甘肽过氧化物酶 (glutathione peroxidage,GPX)和超氧化物歧化酶 (superoxide dism utase,SOD)的活性。 结果 (1)机械通气前 ,早产儿与足月儿 ,低出生体重儿与正常出生体重儿血浆 GPX分别为(90± 6 1)、(116± 5 1)、(94± 6 1)、(113± 5 2 ) k U/ L,CAT分别为 (6 8± 43)、(82± 43)、(6 9± 41)、(84±43) k U/ L,差异无显著性 (P>0 .0 5 ) ,而 SOD在早产儿和低出生体重儿较低 [分别为 (78± 11)、(84±11) k U/ L(P<0 .0 5 )。(2 )机械通气后 ,各组 GPX、CAT、SOD均随通气时间的延长而下降 ,但足月儿、正常体重儿下降幅度较小 ,而早产儿、低出生体重儿下降幅度较大。通气后 3d GPX、CAT、SOD在早产儿分别为 (72± 47)、(5 1± 33)、(5 3± 9) ,足月儿分别为 (10 2± 49)、(78± 37)、(87± 11) ,低出生体重儿分别为 (70± 48)、(4 9± 31)、(5 1± 9) ,正常出生体重儿分别为 (10 3± 47)、(74± 2 3)、(85± 12 ) k U/L,早产儿低于足月儿 ,低出生体重儿各项均低于正常出生体重儿 (P<0 .0 1)。

Objective To explore the effect of mechanical ventilation on antioxidant enzyme (AOE) activities in neonatal blofod plasma. Methods Catalase(CAT) glutathione, peroxidase(GPX) and superoxide dismutase(SOD) activities in blood plasma of seventy two neonates before and after mechanical ventilations were measured by enzyme assay. Results (1)Before mechanical ventilation, the difference of GPX?CAT of premature infants and full term infants ,low birth weight infants(LBWI) and normal weight infants was not significant( P >0.05).The difference of SOD was significant( P <0.05).(2)After mechanical ventilation, GPX. CAT, and SOD activities in plasma decreased in each group with the time of mechanical ventilation. The difference was significant( P <0.01). Conclusion The mechanical ventilation decrease AOE activities in neonatus blood plasma and have even more effects on premature infants and LBWI.