可疑肺癌患者纤支镜后痰液标本的p53基因突变检测

Detection of p53 gene mutation of sputum from postbronchoscopy in patients suspected to have lung cancer

目的 探讨纤支镜后痰液标本的ｐ５３基因突变检测用于肺癌早期诊断的可行性。方法 采用聚合酶链反应－单链构象多态性分析（ＰＣＲ－ＳＳＣＰ）银染技术，对２９例怀疑为肺癌患者的痰液标本行ｐ５３基因５－８号外显子突变检测。结果８例患者有ｐ５３突变，突变率为２７．６％，其中以６号外显子突变率最高（１７．２％）。ｐ５３突变率明显高于相应细胞学阳性率（Ｐ＜０．０５）。结论 纤支镜后痰标本ｐ５３突变检测对肺癌的早期诊断具有一定价值，可作为肺癌早期诊断的辅助方法之一。

Objective To ascertain the feasibility of detecting p53 gene mutations for early diagnosis of lung cancer using spu-tum from postbronchoscopy. Methods Point mutations of exon 5~8 of p53 gene were detected in 29 sputum samples of suspected to have lung cancer using silver staining PCR-SSCP. Results p53 gene mutations were found in 8 of 29 patients (27. 6% ). Muta-tion of exon 6 was most common( 17.2%).The p53 mutation rate was highest in the cells from the sputum (P<0.05). Conclu-sion The above results indicate the value of detecting p53 gene mutation for diagnosis of lung cancer using postbronchoscopic spu-tum.