反转录聚合酶链式反应法检测端粒酶催化蛋白亚单位mRNA

Detecting the mRNA of hTERT by RT-PCR

目的 研究端粒酶催化蛋白亚单位（ｈＴＥＲＴ）ｍＲＮＡ在肿瘤组织中的表达情况，建立一种恶性肿瘤诊断的新方法。方法 提取肿瘤组织总ＲＮＡ，用反转录聚合酶链式反应（ＲＴ－ＰＣＲ）法进行检测。结果７６例各型肿瘤组织中６６例阳性，５例恶性胸腹水全部阳性。结论 ＲＴ－ＰＣＲ法检测 ｈＴＥＲＴ ｍＲＮＡ是一种灵敏、简便的恶性肿瘤辅助诊断的新方法。

Objective To study the expression of the mRNA of the catalytic protein subunit of telomerase (hTERT) in tumor tissues and to develop a novel method to diagnose malignant tumors. Methods Total RNAs of tumor tissues were extracted and were detected by RT-PCR. Results In 76 cases of tumor tissues, 66 cases were positive, and 5 cases of malignant pleural and peritoneal effusions were all positive. Conclusion Detection of the mRNA of hTERT by RT-PCR is a sensitive and simple method to diagnose malignant tumors.