乙肝疫苗免疫前后淋巴细胞表型研究

Analysis of human T-ccll antigen receptor variable β gene usage following vaccination with HBsAg

目的 了解乙肝疫苗（ＨＢｓＡｇ）免疫后健康成人外周血Ｔ淋巴细胞的表型变化，分析表型变化和是否产生抗体应答或弱应答的关系。方法 应用间接免疫荧光法原理和ＦＡＣＳｃａｎ流式细胞仪分析了免疫后健康成人外周血Ｔ淋巴细胞的表型变化；同时应用ＥＬＩＳＡ原理，检测了免疫后ＨＢＶ相关抗体。结果和结论 免疫后ＣＤ３＋细胞和ＣＤ４＋细胞活化增生，提高了机体的免疫调节能力；同时测定了ＨＢｓＡｂ的阳性率。

Objective To study Tc subsets with some CD of PBLs before and after invaccination of HBsAg. Methods Tc subsets with some CD of PBLs before and after invaccination of HBsAg were investigated through FACs (fluorescent activated cell sorter) andHBV- related antibadies were examined by ELISA. Results The results showed that significant increases were found in CD3+ % , CD4+ % , CD4+ /CD8+ postimmunization. Conclusion The results indicate that postimmunization Tc was activated and proliferation ability of immunoregulation was increased. And positive rate of HBsAb is 73% after vaccination.