新疆维吾尔族乙型肝炎病毒慢性无症状携带者的乙型肝炎病毒基因型测定

Determination of HBV genotypes in chronic asymptomatic HBV carriers of Uigur in Xinjiang

目的 :研究新疆维吾尔族乙型肝炎病毒 (HBV)慢性无症状携带者的HBV基因型分布。方法 :利用限制性片段长度多态性 (RFLP)分析技术 ,由限制性内切酶AvaⅡ和DpnⅡ作用于HBVDNA前S区扩增片段建立的简便的HBV基因型分型方法 ,对5 2例HBeAg阳性的新疆维吾尔族HBV慢性无症状携带者的HBV进行基因型测定。结果 :D基因型 5 7.7% (30 / 5 2 ) ,C基因型30 .8% (16 / 5 2 ) ,B基因型 11.5 % (6 / 5 2 ) ,无A、E和F型。结论 :新疆维吾尔族HBV慢性无症状携带者的HBV基因型以D型为优势 ,为我国HBV基因型分布提供了新的资料 。

Objective:To study the distribution of HBV genotypes in chronic asymptomatic HBV carriers of Uigur in Xinjiang.Methods:A simplified RFLP method was established with AvaⅡ and DpnⅡ at amplified segment of the pre-S of HBV DNA,the method was applied to 52 serum samples from HBeAg positive chronic asymptomatic HBV carriers. Results: The genotype D was detected in 57.7%(30/52)of subjects,genotype C in 30.8%(16/52) and genotype B in 11.5% (6/52).There were no genotypes of A?E and F. Conclusion:HBV genotype D is the predominant genotype in chronic asymptomatic HBV carriers of Uigur in Xinjiang.This finding provides a new information for understanding the distribution of HBV genotype in China and a basis to establish Chinese gene bank.