煤尘对人红细胞谷胱甘肽和碱性磷酸酶活性的影响

Effect of Coal-dust on GSH Content and ALP Activity in RBC of Patients with Pneumoconiosis

目的 :为了解煤尘对人血清红细胞谷胱甘肽 (GSH)和碱性磷酸酶 (ALP)活性的影响。方法 :用生物化学方法对 4 8名煤工尘肺 (CWP)患者及 4 0名健康对照者红细胞谷胱甘肽和碱性磷酸酶活性 (ALP)进行了测定。结果 :煤工尘肺患者血清红细胞GSH、ALP含量明显高于对照组 (P <0 0 5或P <0 0 1 ) ,且随着煤工尘肺病程发展和工龄不同 ,上述指标改变程度亦不相同 (P <0 0 5 )。结论 :接触煤尘可使红细胞内氧化代谢产物蓄积 ,从而使其活性、功能活跃 。

Objective: To investigate the effect of coal-dust on the content of GSH and activity of ALP in RBC. Methods: Forty eight cases with pneumoconiosis and 40 healthy controls were enrolled and the activity of ALP and content of GSH in RBC were determined chemically. Results: GSH content and ALP activity of RBC in pneumoconiosis patients were significantly higher than that of controls (P<0 05 or P<0 01) and the alteration increased with pneumoconiosis advancing and with length of the time employed as a coalminer (P<0 05). Conclusion: Coal dust exposure results in the formation of anthracosis and the accumulation of oxidative products. To resist the resultant oxidative damages induced by coal dust, the organism in turn develops corresponding anti-oxidative antagonists:increasing GSH and ALP in RBC.