HPLC法测定远志中去羟基远志皂苷元含量

Determination of dehydroxypresenegenin in Polygalae tenuifolia by HPLC

目的 建立远志药材的质量控制方法。方法 采用 HPL C法对远志中去羟基远志皂苷元进行含量测定 ,ODS Hypersil色谱柱 (5μm ,12 5 m m× 4mm ) ;流动相 :甲醇 - 0 .0 5 %磷酸溶液 (74∶ 2 6 ) ;检测波长 2 10 nm ;结果 去羟基远志皂苷元在 0 .5 85～ 11.7μg范围内与峰面积呈良好的线性关系 ,r=0 .99999;平均回收率为 99.2 1% (n=5 ) ,精密度的 RSD为 0 .2 5 % (n=5 )。结论 该方法简便、准确、重现性好 ,可作为远志药材及其复方制剂质量控制标准。

Object To establish an HPLC method for the determination of dehydroxypresenegenin for the quality control of Polygalae tenuifolia Willd. Methods Dehydroxypresenegenin was separated by ODS column (125 mm × 4 mm, 5 μm) with a mobile phase of methanol 0 05% phosphoric acid (74∶26) and detected at a wavelength of 210 nm. Results The calibration curve was linear in the range of 0 585～11 7 μg,with a correlation coefficient of 0 999 99 . The average recovery was 99 21% (n=5), and RSD=0 25% (n=5). Conclusion The method is simple and accurate, with good repeatability and can be used for the quality control of Polygalae tenuifolia Willd and compound preparations of Radix Polygalae.