维拉帕米对胶原基因表达及大鼠腹膜粘连形成的影响

Effects of verapamil on peritoneal adhesion formation and collage ns gene expression

目的 探讨维拉帕米 (Verapamil)在胶原基因表达及大鼠腹膜粘连形成中的作用。方法 通过造成腹壁和盲肠浆膜的均一缺损制成大鼠粘连模型 ;关腹前注入 1ml生理盐水或维拉帕米 ( 0 .0 0 2kg/L) ;术后 7d进行粘连评分 ;采用逆转录 聚合酶链反应 (RT PCR )方法测定各组织标本Ⅰ (α2 )、Ⅲ (α1)型胶原基因mRNA的表达。结果 与正常腹膜相比 ,模型组和生理盐水组粘连组织Ⅰ (α2 )、Ⅲ (α1)型胶原基因mRNA表达显著增强 ( 0 .64± 0 .17至 4.37± 0 .83、3.5 6± 0 .5 7,P <0 .0 1;0 .0 6± 0 .0 2至 0 .39± 0 .12、0 .47± 0 .2 1,P <0 .0 1) ;与模型组或生理盐水组相比 ,维拉帕米组大鼠粘连显著减轻 ,同时Ⅰ (α2 )、Ⅲ (α1)型胶原基因mRNA表达显著减弱 ( 0 .77± 0 .2 1,0 .0 9± 0 .0 3,P <0 .0 1)。结论 粘连形成过程中胶原基因表达增强 ,抑制胶原基因表达能减轻粘连程度。

Objective To investigate the role of ve rapamil in collagens gene expression and peritoneal adhesion formation.Methods The uniform defects on the peritoneal wall and ce cal surface were developed to establish the rate adhesion model.Prior to complet e closure,1ml of normal saline or verapamil (0.002kg/L) was instilled into the abdominal cavity of the normal saline group or the verapamil group respectiv ely.The severity of adhesions was graded by a blinded observer one week after op eration.The mRNA expression of Types Ⅰ (α_2) and Ⅲ (α_1) collagens in the intact and adhesive tissues was detected by using reverse transcription-polymer ase chain reaction (RT-PCR) technique.Results Both collagen Ⅰ(α_2) and collagen Ⅲ(α_1) ge ne in adhesive tissues in the model group and the normal saline group showed a sign ificantly increased mRNA expression as compared with that in the intact peritone al in the normal control group (0.64±0.17 to 4.37±0.83,3.56±0.57,P<0.01 ; 0.06±0.02 to 0.39±(0.12),0.47±0.21,P<0.01).The verapamil group had sign ificantly milder adhesions than in the model group or the normal saline group.Th e mRNA expression of both collagen Ⅰ(α_2) and collagen Ⅲ (α_1) gene in adh esive tissues in the verapamil group (0.77±0.21,0.09±0.03,respectively) was significantly decreased as compared with that in the model group or the normal s aline group (P<0.01).Conclusion Collagens gene expression was significantly in creased during the process of adhesion formation.The inhibition of collagens gen e expression ccould significantly reduce the sever ity of adhesion formation.