一氧化氮吸入治疗急性肺损伤的作用机制

The mechanism of NO inhalation in the treatment of acute lung injury

目的 探讨一氧化氮 (NO)对内毒素致伤大鼠急性肺损伤 (ALI)肺组织核因子 KB(NF kB)活性及肿瘤坏死因子 α(TNF α)水平的影响和防治ALI的作用机理。方法 观察内毒素(LPS)和LPS/NO对大鼠血氧分析和肺组织损伤的影响 ,并采用凝胶电泳迁移率改变 (EMSA)法检测肺组织核蛋白提取物中NF kB活性和检测的特异性及酶联免疫检测 (ELISA )测定肺组织匀浆TNF α含量改变。结果 NO吸入后 ,显著抑制LPS静注致大鼠急性肺损伤、肺组织核蛋白NF kB活性的增强以及肺组织匀浆TNF α含量的升高。结论 NO通过抑制NF kB活性 ,下调TNF α等炎症分子表达而发挥抗炎和防治ALI的作用。

Objective To investigate the therapeutic mechanism of NO inhalation in the treatment of acute lung injury (ALI) induced b y LPS.Methods The change of NF-kB activity and the expre ssion levelof tumor necrosis facter-α(TNF-α)in pulmonary tissue of rats with ALI induced byLPS after NO-inhalation was observed by using EMSA and ELISE. Me anwhile,the pathologic changes were examined under optical microscopy.Results Both NF-kB activity and TNF-α content in pulmo nary tissue of rats with ALI after NO-inhalation were decreased signficantly.Conclusion NO-inhalation inhibited NF-kB activity and d ownregulated the expression of TNFαin lung tissue of rats with ALI, suggesting that NO inhibited the inapprepricate activation of NF-kB and overexpression of TNF-α and might play an antiinflammatory role in ALI induced by LPS.