食管癌和贲门癌组织中错配修复基因表达的研究

Study on the expression of mismatch repair genes in human esophageal cancer and cardiac carcinoma

目的 研究错配修复基因 (MMR)表达与食管癌和贲门癌癌变的关系和甲基苄基亚硝胺 (NMBzA)对其影响的研究。方法 采用多重逆转录聚合酶链反应 (RT -PCR)。结果 31例食管癌hMSH2基因表达明显降低占 2 2 .6 % ;hMLH1基因表达明显降低占 6 .45 % ;未发现hPMS2 ,GTBP和hPMS1基因表达降低。 1 1例食管癌旁组织分析 :hMSH2 ,hPMS2 ,GTBP ,hMLH1基因表达明显降低占 9.0 9% ;未发现hPMS1基因表达明显降低。 1 2例贲门癌组织分析 :hMSH2基因表达明显降低占 41 .7% ;GTBP ,hMLH1 ,hPMS1基因表达明显降低占 8.3 % ;未发现hPMS2基因表达明显降低。 8例贲门癌旁组织分析 :3例hMSH2基因表达明显降低 ;1例hPMS2基因表达明显降低 ;3例hMLH1基因表达明显降低 ;未发现GTBP ,hPMS1基因表达明显降低。对NMBzA致癌物诱发的人胎儿食管上皮癌细胞株分析发现 :hMLH1和hPMS1基因低表达 ;hMSH2 ,hPMS2 ,GTBP基因不表达。结论 食管癌和贲门癌癌变可能与错配修复基因失活有关 ;甲基苄基亚硝胺能引起错配修复基因表达异常。

Objective To elucidate the relationship between expression of mismatch repair genes(MMR) and the corcivegenesis of esophageal cancer or cardiac carcinoma and to study the effect of N Nitrosomethylbenzylamine(NMBzA) on the expression of mismatch repair genes. Methods Expression of mismatch repair genes were measured by multiplex reverse transcription polymerase chain reaction. Results Of the 31 specimens of esophageal cancer, 7(22.6%) had greatly reduced expression of hMSH2; 2(6.45%) had greatly reduced expression of hMLH1; but none had greatly reduced expression of hPMS2,GTBP and hPMS1. Of the 11 specimens of tumor adjacent tissue from patients with esophageal cancer, 1 (9.09%) had greatly reduced expression of hMSH2, hPMS2, GTBP and hMLH1; none had greatly reduced expression of hPMS1. Of the 12 specimens of cardiac carcinoma, 5(41.7%) had greatly reduced expression of hMSH2, 1(8.3%) had greatly reduced expression of GTBP, hMLH1 and hPMS1; none of had greatly reduced expression of hPMS2. Of the 8 specimens of tumor adjacent tissue from patients with cardiac carcinoma, 3 had greatly reduced expression of hMSH2; one had greatly reduced expression of hPMS2; none had greatly reduced expression of GTBP and hPMS1, 3 had greatly reduced expression of hMLH1. One cell line of human fetal esophageal carcinoma induced by NMBzA, had no detectable expression of hMSH2, hPMS2 and GTBP; but had low expression of hMLH1 and hPMS1. Conclusion These data suggest that mismatch repair genes may play a role in the corcivegenesis of human esophageal cancer and cardiac carcinoma and NMBzA can effect on the expression of mismatch repair genes in human esophageal cancer.