p53、血管内皮生长因子在大肠癌组织中的表达与血管生成

The expression of p53 and vascular endothelial growth factor and their relationship to neoangiogenesis in human colorectal cancer

目的 探讨p53、血管内皮生长因子 (VEGF)在大肠癌组织中的表达及其与血管生成的关系。方法 利用免疫组化SABC法 ,对 1 0 6例大肠癌组织及 2 0例正常大肠组织中的 p53、VEGF的表达及微血管密度 (MVD)进行研究。 结果 p53、VEGF的表达与肿瘤的分化程度及Dukes分期无明显相关性 (P >0 .0 5 )。p53表达阳性或VEGF表达阳性的大肠癌组织MVD明显高于p53表达阴性 (P <0 .0 1 )或VEGF表达阴性者 (P <0 .0 1 )。p53表达阳性的大肠癌组织中VEGF的表达阳性率显著高于 p53表达阴性者 (P <0 .0 1 )。结论 p53、VEGF在大肠癌的发生和发展中起着重要作用 ,可反映大肠癌的恶性程度和进展情况并作为预后的指标 。

Objective To investigate the expression of p53 vascular endothelial growth factor (VEGF), and their role of in the regulation of angiogenesis in human colorectal cancer. Methods SABC immunohistochemical method was used to investigate the expression of p53, VEGF and microvessel density in 80 cases of colorectal cancer and 10 cases of normal colorectal tissue. Results The p53 was mainly localized in nuclei of tumor cell, VEGF was mainly localized to the cytoplasm and membrane of tumor cell. The positive rate of p53 and VEGF in colorectal cancer was significantly higher than that in normal colorectal tissue ( P <0.01), and they were not associated with the differentiation and Dukes grade of carcinoma ( P<0.05 ). The MVD in p53 or VEGF positive tumor was significantly higher than that in colorectal cancer out expression of p53 ( P<0.01 ). Conclusion p53 and VEGF play a key role in occurrence and development of colon cancer and serve as an indicator of malignancy and development of colon cancer. Nuclear p53 accumulation may associate with tumor angiogenesis through VEGF up-regulation.