急性白血病患者血清一氧化氮合酶水平的变化

Serum Levels of Nitric Oxide Synthase Activity in Patients with Acute Leukemia

目的 :探讨急性白血病患者血清一氧化氮合酶 (NOS)水平的变化及其意义。方法 :采用比色法测定 5 0例急性白血病患者血清 NOS的活性。结果 :血清 NOS活性在不缓解 (NR)或复发的急性淋巴细胞白血病 (AL L )、急性髓性白血病 (AML)患者分别为 (5 .39± 2 .38) u/ ml(n=10 )、(4 .2 0± 1.12 ) u/ ml(n=17) ,与部分缓解 (PR) /完全缓解 (CR) AL L的 (2 .94± 1.0 ) u/ m l(n=14)、AML的 (3.0 3± 1.2 1) u/ ml(n=17)及正常对照组的 (2 .38± 0 .74) u/ ml(n=2 0 )分别比较 ,除 AML 组的 NR/复发同 PR/ CR相比的差异呈显著性意义外 (0 .0 1<P<0 .0 5 ) ,其余差异均有非常显著性意义(P<0 .0 1) ;血清 NOS活性与急性白血病疗效呈负相关关系 (χ2 =12 .2 3,P<0 .0 1)。结论 :血清 NOS活性水平升高与急性白血病病理活动状态、化疗效果及预后不良有关。

Objective:To study the significance and serum levels of nitric oxide synthase activity (NOS) in patients with acute leukemia.Methods:The activity of serum NOS was measured by spectrophotometry in 50 patients with acute leukemia.Results:The levels of serum NOS activity were obviously higher in non remission(NR)/relapsed patients with acute lymphoblastic leukemia(ALL) or acute myeloid leukemia(AML) than those in control subjects or completely remission (CR)/partial remission (PR)(ALL or AML)group(±s:5.39±2.38,4.20±1.12,2.38±0.74,2 94±1.0,3.03±1.21μ/ml,respectively; P <0.01 in each comparison,except 0.05< P <0.01 in NR/relapsed versus CR/PR group of AML).Moreover,the NOS activity could be correlated with NR/relapsed acute leukemia(χ 2=12 23, P <0.01).Conclusion:Determination of NOS activity might be a marker associated with leukemia activity,to be used in evaluation and/or monitoring of chemitherapy response and outcome.