大鼠胰岛分离、肾被膜下移植方法的改进

Modified islet isolation and transplantation method in rats

目的 改进大鼠胰岛分离和移植方法。方法 在静止消化法和借助黄色吸头移植法的基础上加以改进的方法。结果 ①胰岛分离所需时间为 16 4 0 0± 11 74min。②纯化后胰岛收获量为 32 0 5 0± 44 2 5个 /只。③胰岛纯度为 95 90± 0 99(% )。④胰岛移植所需时间为 2 7 6 0± 1 71min。⑤同系胰岛移植后 1～ 2d ,糖尿病大鼠血糖下降到2 0 0mg/dl以下。⑥胰岛计数时使用显微镜的磨砂反光镜面 ,可清楚区分胰岛、外分泌组织、淋巴结。结论 ①改进后的分离法和移植方法更加简便 ,易于掌握。

Objective This study aimed to improve methods of islet isolation and transplantation in rats.Methods Modified stationary digestion method and islet transplantation using yellow tip method was used in this study.Results ①Time of islet isolation was 164.00±1.74min ②The yield of pureislets was 320.50±44.25/rat ③Purity of islets was 95.90±0 99(%) ④Time of islet transplantation was 27.60±1.71min.⑤At 1～2 days after syngenic islet transplantation,blood glucose level of diabetic rats fell under 200mg/dl.⑥Islets,exocrine tissue and lymph nodes were clearly divided,when ground reflector of microscope was used.Conclusion ①Islet isolation and transplantation method after improvement was more convenient.②Ground reflector of microscope was useful for observation of islets.