双氯芬酸钠脂质体凝胶剂的研制

Study on diclofenac sodium liposome hydrogel

目的 :研制双氯芬酸钠脂质体凝胶 ,并进行评价。方法 :运用薄膜蒸发法制备了双氯芬酸钠脂质体 ,然后用卡波姆 (Carbopol)为基质制得凝胶。并建立了一阶导数紫外分光光度法进行测定。结果 :测得样品低、中、高 3个浓度的平均回收率分别为 (10 1 1± 0 86 ) % ,(99 90± 1 0 4 ) %和 (10 0 5± 0 91) %。脂质体经凝胶柱分离后测得平均包封率为 4 3 6 % ,经库尔特计数器测得平均粒径为 1 37μm。凝胶分别于冰箱 (4℃ )和室温下密封保存 0 5、1、3、6mo ,6mo后的包封率下降分别为 4 4 %、6 8%。结论 :经初步评价 。

OBJECTIVE:To prepare a liposome hydrogel of diclofenac sodium(DCS) and evaluate it. METHODS:The DCS liposomes were prepared by the conventional thin film hydration method at first, to which the carbopol was added as a support base for preparation of the hydrogel of DCS. An one-order derivative UV spectrophotometry was established to evaluate the DCS in hydrogel.RESULTS:The average recoveries of three samples were (101 1±0 86)%,(99 90±1 04)%和(100 5±0 91)%, respectively and the average encapsulation efficiency of liposomes is 43 6% after separated by gel minicolumn. The particle sizes of liposomes were determined by a coulter counter and d 50 was 1 37μm. In stability of test, the hydrogel was stored for 0 5, 1, 3 or 6 months at 4℃ or at room temperature, and the encapsulated efficiency of liposomes was decreased by 4 4% or 6 8% respectively after six months.CONCLUTION: It is feasible to prepare a liposome hydrogel of DCS after an initial evaluation.