大鼠短暂性脑缺血后葡萄糖转运子3表达变化

Alternations of glucose transporters-3 expression after transient cerebral ischemia

目的 研究大鼠短暂性脑缺血葡萄糖转运子３（ＧＬＵＴ３）转录水平表达规律。方法 用插线法建立大鼠短暂性脑缺血模型，剥取缺血半暗带及中心区皮质组织，采用半定量逆转录－聚合酶链式反应（ＲＴ－ＰＣＲ），测定不同再灌注时间ＧＬＵＴ３ｍＲＮＡ水平的变化。结果 再灌注３ｈ缺血半暗区ＧＬＵＴ３ｍＲＮＡ３ｈ升高，４８ｈ达到高峰，再灌注１周后仍高于正常。结论 再灌注后，缺血半暗带ＧＬＵＴ３的表达明显上调，有可能是机体抗损伤性反应。

Objective To investigate the changes of glucose transporter-3 (GLUT3) transcription level at different reperfusion times in rats ischemic penumbra and core of cortices. Methods Focal ischema models of middle cerebral artery occlusion (MCAO) were achieved by inserting nylon surgical thread through the internal carotid artery(ICA) into the anterior cerebral arterv(ACA). After 2h of left MCA occlusion, the thread was re- moved to allow reperfusion. Brain samples were harvested from ischemic penumbra and core of cortices. The ex- pression of GLUT3 mRNA was assessed by RT-PCR. Results GLUT3 mRNA in the penumbra regions slightly increased at 3h of reperfusion and reached a peak at 48h. In 1 week following reperfusion. it was still higher than normal level. As for ischemic core regions. GLUT3 mRNA had a short time increase from 3h to 8h of reperfusion and rapidly decrease to low level. Conclusion GLUT3 mRNA expression was notably up-regulated in the penum- bra regions after focal cerebral ischemia reperfusion. It may be protective reaction against ischemic insults.