C肽、胰岛素在实验性NIDDM大鼠GT实验中的变化

The Changes in Levels of C-P and Insulin in Glucose Tolerance Test in Rats with Experimental Non-insulin Dependent Diabetes Mellitus

为探讨C肽 (C -P)、胰岛素 (INS)在非胰岛素依赖型糖尿病 (NIDDM )模型糖耐量 (GT)实验中的变化 ,用小剂量尾静脉注射链脲佐菌素 (STZ)加喂高热量饮食形成NIDDM鼠模型 ,对GT实验中C -P、INS的变化进行观察。结果显示 :与对照组比 ,实验组INS升高的高峰时间是在两小时 ,C -P同INS反映一致 ,高峰推迟 (P <0 .0 5 )。提示 :血中C -P测定能准确反映胰腺内生INS水平 。

The changes in levels of C-P and insulin were investigated in the GT test in rats with non-insulin dependent diabetes mellitus. In order to establish a model of non-insulin dependent diabetes mellitus (NIDDM), we injected rats with small dose streptozocoi (i.v.). Two weeks after the injection, the rats developed impaired glucose tolerance(IGT). Then, they were fed with high energy diet for eight weeks to form NIDDM. The results showed that the highest peak time of C-P and insulin in NIDDM was remarkably later than that in normal subjects, the highest peak time was in two hours ( P <0.05). The data suggest that level of C-P could accurately respond to level of insulin, and this experimental non-insulin dependent diabetes mellitus model is ideal.