转化CaMV基因Ⅵ芸薹属蔬菜植株抗病性鉴定及其遗传分析

Diagnosis and Genetic Analysis of Resistance to Cauliflower Mosaic Virus in Brassica Crops Which Was Transformed with CaMV Gene VI

以转化CaMV弱株系Bari 1基因Ⅵ的大白菜、菜薹、紫菜薹和花椰菜植株为试材 ,研究了CaMV弱株系Bari 1基因Ⅵ所提供的遗传工程交叉保护及其遗传规律。结果表明 ,在转化CaMV弱株系Bari 1基因Ⅵ的大白菜、菜薹、紫菜薹和花椰菜植株中 ,4 8.0 8%对CaMV强株系CABB BJI具有较强的抗性 ,5 1.92 %表现为敏感。抗性基因 (CaMVBari 1基因Ⅵ )在自交代 (S1)中大多数 (76 % )表现为典型的孟德尔单基因显性遗传 ,部分株系的抗性出现了15∶1,1∶1,1∶3和

The genetically engineered cross protection which was supplied by weak strain Bari 1 gene VI of cauliflower mosaic virus(CaMV),and its genetic regularity were studied with Brassica crops( Brassica campestris ssp. rapifera,B.campestris ssp. pekinensis,B.campestris var. parachinensis,B.campestris var. purpurea,B.juncea,B.oleracea var. botrytic )which were transformed with CaMV gene VI.The results showed that 48.08% of transformed Brassica plants was resistance to CaMV strain CABB BJI and 51.92% of them was susceptible to CaMV CABB BJI.The virus resistant gene(CaMV Bari 1 gene VI)in 76% of first selfed progeny presented typically Mendelian single dominance gene inheritance.The ratio of virus resistant to virus susceptible plants in some of first selfed progeny presented non Mendelian inheritance,such as,15∶1,1∶1,1∶3 and 1∶95.