华南地区新城疫病毒WS_(99)株HN基因片断的克隆和序列分析

Cloning and Sequencing of HN Gene of NDV WWS_(99) Strain Isolated in South China

用RT -PCR一步法扩增了华南地区野毒NDVWS99株的HN基因 890bp片断 ,并对其进行克隆、核苷酸测序及同源性分析 结果表明 :WS99株的HN基因与其他 2 9株NDV的核苷酸同源性在 88.0 %～ 99.8%之间 ,氨基酸同源性在 90 %～ 97%之间 鸡源NDVWS99株与鹅源副粘病毒GPMV株有极高的同源性 ,高达 99.8% 该毒株与TEX/ 48和B1/ 47一样 ,在HN基因 130 1bp处由G突变为A ,对应氨基酸由V突变为I,但其半胱氨酸位点和个数没有改变 ,且所克隆的目的片段中含有HN基因上 5个凝血抗原位点其中的

The HN gene of NDV WS 99 strain isolated from South China was amplified by one step nested RT-PCR. The HN gene was cloned sequenced and analyzed homologically. It was found that the nucleotide sequence homology of WS 99 strain with other 29 NDV strains was between 88.0% to 99.8%. Their amino acid sequence showed 90% to 97% homology. Significantly, the NDV WS 99 strain isolated from chicken and the GPMV, a paramyxovirus isolated from goose in South China exhibited the highest homology of nucleotide which was 99.8%. The WS 99 strain had a nucleotide mutation at the 1 301 bp site of the HN gene, with Guanine replacing Adenine. Thus the amino acid code mutated from V to I. but there were no changes in the quantity and loci of cysteine in the HN. The cloned gene of WS 99 strain contained 4 of the 5 haemagglutinating antigenic domains of the HN gene.