肝移植对胃功能的影响及意义

Effects of liver transplantation on gastric function and its significance

目的:观察肝移植前后胃功能及组织结构的变化情况.方法:制备400mL·L-1CCl4中毒性肝硬化大鼠模型(n=40),采用“二袖套法”进行肝移植,利用滴定法测定大鼠胃酸,应用放免分析法,以SPECT显像用的核素99Tcm为标记,采取液相胃排空的方法,分析肝移植前后大鼠胃排空的情况.经组织学和电镜切片观察,肝移植前后胃黏膜的组织结构和细胞结构的变化.结果:肝移植前,肝硬化大鼠的胃酸分泌减低(从40.7±8.8mmoL·L-1至28.5±4.6mmoL·L-1)?胃排空时间明显延长(胃排空率从89.3±6.8%至78.2±6.5%),胃黏膜的组织结构受到损害(胃黏膜溃疡指数从4.3±1.2至34.8±4.6).肝移植后,随着时间的不断延长,大鼠胃酸分泌增加(从28.5±4.6mmoL·L-1至39.4±7.6mmoL·L-1)?胃排空状况明显改善(胃排空率从78.2±6.5%至88.1±6.7%),胃黏膜组织结构逐渐恢复(胃黏膜溃疡指数从34.8±4.6至10.5±2.7).结论:肝移植可使因肝功能衰竭而导致的胃功能和胃黏膜的损害得到很好的改善.

AIM:To observe the changes of rats gastric function and tissu e structure before and after liver transplantation. METHODS:Hepatic cirrhosis model was established in rats ( n=40) by subcutaneous injections of 400mL·L-1 carbon tetrachloride. Rat liver transplantation was made with two-cuff technique. Rat gastric acid wa s determined with titration. Gastric emptying was analyzed with radioimmunoassay, by using nuclide 99Tcm and gastric emptying of liquids before and after liver transplantation. The changes of gastric mucosa tissue structure and cell struct ure were observed with histological method and electron microscope before and af ter liver transplantation. RESULTS:The exudation of gastric acid decrease (from 40.7±8.8mm oL·L-1 to 28.5±4.6mmoL·L-1), the time of gastric emptying prolo nged (the gastric emptying rate from 89.3±6.8% to 78.2±6.5%) and the structure of gastric mucosa was damaged (ulcer index, UI: from 4.3±1.2 to 34.8±4.6) bef ore transplantation. After transplantation, the exudation of gastric acid increa sed (from28.5±4.6mmoL·L-1 to 39.4±7.6mmoL·L-1), gastric empty ing was improved significantly (the gastric emptying rate from 78.2±6.5% to 88. 1±6.7%) and the tissue structure of gastric mucosa resumed (UI: from 34.8±4.6 to 10.5±2.7) after transplantation. CONCLUSION:Transplantation can improve the destruction of gastr ic function and gastric mucosa tissue owing to liver function failure.