几种抗癌药物诱导胰腺癌细胞凋亡能力的比较

Comparing the apoptotic inducing ability of some anti-tumor drugs to pancreatic cancer cell

目的:比较抗癌药物诱导胰腺癌细胞凋亡的能力,以期找到一种疗效较好的化疗药物.方法:根据临床常用的最高及最低剂量,分别将羟基喜树碱?三氧化二砷?紫杉醇?全反式维甲酸?奥曲肽及5-Fu与胰腺腺泡癌细胞SW1990系共同培养6?12?24h,利用光镜及电镜下的形态学方法及脱氧核糖核苷酸末端转移酶介导的缺口末端标记法(TUNEL)确定细胞凋亡,并利用AO-EB双重荧光染色法及PI和Annexin-VFITC双重标记下的流式细胞术定量测定细胞凋亡率.结果:上述药物均可诱导SW1990细胞凋亡,凋亡诱导能力由强到弱依次为紫杉醇?5-氟尿嘧啶?羟基喜树碱?三氧化二砷?全反式维甲酸和奥曲肽.各药物的凋亡诱导能力呈剂量和时间的依赖性,其中紫杉醇的作用最强且最快.结论:紫杉醇可能是一种疗效较好的胰腺癌化疗药物.

AIM:Comparing the apoptotic inducing ability of some anti-tu mor drugs to pancreatic cancer cell, in order to find a better chemothera- peutic medicine to pancreatic cancer. METHODS:According to the highest and lowest doses commonly used in clinic, culturing the pancreatic cancer cell line SW1990 with hydroxycamptoth ecin, arsenic trioxide, taxol, all-trans retinoic acid, octreotide and 5-Fu fo r 24 hours respectively, using morphologic methods under light or transmitted el ectronic microscopy and terminal deoxynucleotidyl transferase mediated nick end labeling (TUNEL) methods to demonstrate cell apoptosis, and using AO-EB double fluorescence stain and PI-Annexin V-FITC double labeling flow cytometry to det ermine the cell apoptotic percentage. RESULTS:These medicine all can induce pancreatic tumor cell line SW1990.apoptosis, the apoptotic inducing ability ranks from higher to lower is taxol,5-Fu,hydroxycamptothecin, arsenic trioxide, all-trans retinoic acid and octreotide. And the apoptotic inducing ability of each medicine depends on dosag e and time, Taxol has the most quick and strong effect. CONCLUSION:Taxol may be a better chemotherapeutant to pancreatic cancer.