血管紧张素转化酶基因多态性与口服避孕药对妇女脑卒中影响的研究

The Research in Risk of Stroke:Angiotension-Converting Enzyme Gene Polymorphism and Oral Contra-ceptives.

目的:初步探讨血管紧张素转化酶(ACE)基因插入/缺失(I/D)多态性及复方口服避孕药(COC)使用对脑卒中的影响。方法:收集女性脑卒中患者,利用PCR技术和扩增片段长度多态性(AFLP)的方法检测ACE基因多态性。根据高血压的有无将患者分成两组,按病例-病例研究的方法进行探讨。结果:无论是单独考虑服药或是单独考虑携带D型等位基因,其对高血压均没有显著性影响(P>0.05)。但是携带D型等位基因的妇女,如果同时服药15年以上,发生高血压的危险性将有显著性增加(OR=5.71,P=0.03)。结论:携带D型等位基因和长期服用COC(15年以上)可能会增加高血压的发生。提示对妇女服药前进行遗传危险因素筛选和服药时血压监测的必要性。

Objective:To assess the effects of combined oral contraceptives(COC) and angiotension- converting enzynme(ACE) geneinsertion/deletion (I/D) polymorphism on stroke. Mothods: ACE genepoly-morphism was detected by polymerase chain reaction(PCR) and amplification fragment length polymorphism (AFLP).The patients were divided into two groups by with hypertension or not, and the interaction between using COC and carrying D allele was analyzed by case-case study. Results:There were no significant effects on hypertension either COC or Dallele(P >0. 05) ,but if the women with D allele had a history of using COC for more than 15 years, the risk of hypertension would be significantly high (OR = 5.71, P = 0.03). Conclusion: D allele and long-term use of COC(more than 15 years)maybe accelerate the onset of hypertension. It seems necessary to examine women's genetic risk factor before using COC and monitor their blood pressure during using COC.