肝癌患者血浆VEGF、sVCAM和bFGF浓度的研究-与术后短期复发的相关性

STUDY OF PLASMA CONCENTRATION OF VEGF,sVCAMAND bFGF IN THE PATIENTS WITH HEPATOCELLULARCARCINOMA-THE RELATIONSHIP WITH EARLYRECURRENCE

目的 检测VEGF、sVCAM和bFGF在不同人群特别是肝癌患者血浆中的浓度,并研究上述因子在预测肝癌术后复发的价值。方法 采用ELISA方法检测96例肝癌患者,以及健康人、慢性肝病患者和肝脏血管瘤患者血浆中VEGF、sVCAM和bFGF浓度。结果 肝癌患者血浆VEGF、sVCAm和 bFGF浓度分别为230.4pg/ml、767.lng/ml和23.lpg/ml,均高于其他人群,仅在慢性肝病患者血浆sVCAM浓度高于肝癌(1584.1ng/ml)。在肝癌患者中,VEGF浓度在肿瘤直径＞5cm、有癌栓或镜下癌栓的患者体内水平较高(270pg/ml vs．171pg/ml;389pg/ml vs．212pg/ml,366.0pg/ml vs.210.9pg/ml, P＜0.05); sVCAM则在乙肝e抗原阳性或肝硬化结节＞3mm的肝癌患者体内较高(1017.7ng/ml vs.706ng/ml,994.3ng/vs．636.6ng/ml,P＜0.05);bFGF在大肝癌、肿瘤包膜不完整的肝癌患者体内较高(36.7pg/ml vs．24.4pg/ml,42.4pg/ml vs．22.lpg/ml,P＜0.05)。比较肝癌切除术后短期复发与无复发病例发现,术前VEGF在复发病例中的浓度高于无复发病例(387.0pg/ml vs. 163.3pg/ml,P＜0.05)。结论VEGF、sVCAM和bFGF均与反映肝癌侵袭性的临床病理指标有关,sVCAM则很可能反映了肝实质的慢性炎症,而VEGF预测肝癌术后短期复发的最有价值。

ve To detect the plasma concentration ofVEGF,sVCAM-l and bFGF in the patients with hepatocellularcarcinoma (HCC) and to evaluate the predictive value for the earlyrecurrence. Methods The plasma concentrations of VEGF,sVCAM and bFGF were measured by ELISA in healthy people, andpatients with chronic hepatitis, hemangioma and HCC.ResultsTHe plasma concentrations of VEGF.sVCAM and bFGF in HCCpatients were 230.4 pg/ml,767.l ng/ml and 23.1 pg/ml,whichwere higher than those in other group, except sVCAM in chronictepatitis patients were higher than that in HCC patients.VEGF levelwas higher in the patients with big tumor (>5 cm)、tumor thrombior microscopic thrombi(270 pg/ml vs l71 pg/ml; 389 pg/ml vs2l2 pg/ml; 366.0 pg/ml vs 2l0.9 pg/ml, P<0.05); sVCAMlevel was higher in the patients with positive HBeAg or macronodularcirrhosis (l017.7 ng/ml vs 760 ng/ml;994.3 ng/ml vs 636.6 ng/ml, P>0.05);bFGF level was higher in patients with big tumor oruncomplete tumor capsule (36.7 pg/ml vs 24.4 pg/ml;42.4 pg/ml vs 22.l pg/ml, P<0.05). In patients with early recurrenceafter resection, VEGF, svCAM level was higher than those withoutearly recurrence (387.0 pg/ml vs l64.3 pg/ml, P<0.05).Conclusion VEGF, sVCAM and bFGF were all related with theinvasiveness of HCC, however sVCAM was possibly related withchronic hepatitis; VEGF was a Predictive factor for the earlyrecurrence of HCC.