胰管刷检标本p53蛋白检测在胰腺癌诊断中的价值

Diagnostic significance of detecting p53 protein in cytologic specimens by endoscopic pancreatic duct brushing for pancreatic cancer

目的 探讨胰管刷检标本 p5 3蛋白检测在胰腺癌诊断中的价值。 方法 应用免疫组化法检测 2 6例胰腺及壶腹疾病患者胰管刷检标本 p5 3蛋白的表达 ,并与常规细胞学检查作比较。 结果苏木精 伊红染色常规细胞学检查诊断胰腺癌的敏感性为 5 3% ,特异性为 10 0 % ,准确性为 70 %。胰管刷检标本 p5 3蛋白检测诊断胰腺癌的敏感性为 5 9% ,特异性为 10 0 % ,准确性为 74%。二者联合诊断胰腺癌的敏感性为 71% ,特异性为 10 0 % ,准确性为 81% ,与单项细胞学检查相比差异有非常显著性 (P<0 .0 1)。结论 胰管刷检标本细胞学检查的同时 ,进行 p5 3检测可提高胰腺癌的诊断率 ,有助于胰腺良、恶性疾病的鉴别。

Objective To investigate diagnostic significance of detecting p53 protein in cytologic specimens by endoscopic pancreatic duct brushing for pancreatic cancer. Methods p53 protein in cytologic specimens was detected by immunohistochemistry, and the resules were compared with cytologic specimens by HE staining. Results Sensitivity, specificity, and accuracy of HE staining for pancreatic cancer is 53%, 100% and 70% respectively. Sensitivity, specificity, and accuracy of detecting p53 protein for pancreatic cancer is 59%, 100%, 74% respectively. Sensitivity, specificity, and accuracy of HE staining and detecting p53 protein for pancreatic cancer is 71%, 100%, 81% respectively. Conclusions HE staining combined with detecting p53 protein in cytologic specimens by endoscopic pancreatic duct brushing is a useful stool for diagnosis of pancreatic cancer. It is helpful to differentiate benign diseases of pancreas from malignant tumor of pancreas.