胆囊癌组织中CD44v6抗原表达的定量研究

Quantitative study on the expression of CD44v6 antigen in human gallbladder carcinoma

目的 探讨CD44v6抗原的表达及含量与胆囊癌的临床病理意义。方法 应用微波 LSAB免疫组织化学法和图像分析技术 ,检测 45例胆囊癌、17例胆囊腺瘤和 10例慢性胆囊炎组织中CD44v6抗原表达及含量。结果 CD44v6抗原表达阳性率及阳性细胞积分光密度值 ,在胆囊癌中显著高于胆囊腺瘤和慢性胆囊炎 (P <0 .0 5 ,P <0 .0 1) ;胆囊癌Ⅱ、Ⅲ级显著高于Ⅰ级 (P <0 .0 5 ,P <0 .0 1) ;有转移的肿瘤显著高于无转移的肿瘤 (P <0 .0 5 ,P <0 .0 1)。结论 CD44v6抗原表达与胆囊癌的发生、细胞增殖、分化和转移相关。对判断胆囊癌的恶性程度、预测生物学行为和预后具有一定临床意义。

Objective To study the clinicopathological significance of CD44v6 antigen expression and its contents.Methods CD44v6 expression and its contents in 45 cases of gallbladder carcinoma,17 cases of gallbladder adenoma and 10 cases of chronic cholecystitis were evaluated by microwave-LSAB immunohistochemical technique combined with image analysis.Results Positive expression rate of CD44v6 antigen and its integral optic density in gallbladder carcinoma were 76.0%(34/45) and 3.91±3.22 respectively,significantly higher than 29.4%(5/17) and 0.97±0.52 in gallbladder adenoma,and 20%(2/10) and 0.40±0.12 in chronic cholecystitis (P<0.05,P<0.01).In grade Ⅱ～Ⅲ,they were 94.7%(18/19) and 6.32±1.76 significantly higher than 61.5%(16/26) and 2.28±3.32 in grade Ⅰ(P<0.05,P<0.01).In lymph node positive cases,they were 95.8%(23/24) and 6.58±3.42,significantly higher than 52.4%(11/21) and 3.41±2.39 in negative ones(P<0.05,P<0.01).Conclusion CD44v6 expression is correlated with the pathogenesis,differentiation and metastasis of gallbladder carcinomas.It may serve as a biological indicator to predict the malignancy and the prognosis of the patients with gallbladder carcinoma.