PCR-SSCP分析法检测胃癌中p27基因突变

Mutations of p27 gene in human gastric cancinoma

目的 对 5例早期胃癌组织和 32例进展期胃癌及其各自的正常胃粘膜组织 ,进行p2 7抑癌基因的突变检测。方法 应用聚合酶链式反应—单链构象多态性分析 (PCR SSCP)技术进行检测。结果 在早期胃癌组织中检出突变率为 2 0 .0 %( 1/5 ) ,在进展期胃癌组织中的突变率为 2 1.9%( 7/32 )。实验结果表明 ,p2 7基因在早期胃癌与进展期胃癌中均有较高突变频率 ,两者差异无显著性 ;p2 7基因突变在胃癌中与性别、年龄以及肿瘤的位置、组织分化程度、Borrmanm分期和有无淋巴结转移无关 ;p2 7基因外显子 1是该基因突变的集中区域 ,且以第 1-90密码子区域突变率最高 ,p2 7基因外显子 2突变率最低。结论 证实了p2 7基因突变发生于早期胃癌形成阶段 ,属于胃癌发生的早期事件。

Objective To study the mutation of p27 gene in human gastric carcinoma.Methods Five specimens of early gastric carcinomas and 32 specimens of advanced gastric carcinomas and their normal mucosa were analyzed for the p27 gene MCR(mutation cluster region) by PCR-SSCP(single strand conformation polymorphism) technique.Results In 1 out of 5 early gastric carcinomas(20.0%) and 7 out of 32 cases of advanced gastric carcinomas (21.9%),mutations were detected. p27 gene mutation was not related to sex, age , location, histologic features, Borrmanm type and lymph node metastases .In MCR region, 1-90 codons showed the highest mutation frequency, while exon 2 showed a lowest mutation frequency.Conclusion p27 gene mutation detected in early gastric carcinomas indicates that the p27 gene mutation is an early event in gastric carcinogenesis,which is helpful to the diagnosis of human gastric carcinoma.